Pharmacologyonline 2: 74-89 (2008) Bhaumik et al. 74 ANTIOXIDANT AND FREE RADICAL SCAVENGING PROPERTY OF METHANOL EXTRACT OF BLUMEA LANCEOLARIA LEAF IN DIFFERENT IN VITRO MODELS

Uttam Kumar Bhaumik, D Kumar, V Selvan, Prerona Saha, M Gupta, U Mazumder
2008 Pharmacologyonline   unpublished
The defatted methanol extract of Blumea lanceolaria (MEBL) was evaluated for its antioxidant property in various invitro systems. DPPH radical scavenging, nitric oxide radical scavenging, hydroxyl radical scavenging, lipid peroxidation inhibition, reductive ability and estimation of total phenolics were evaluated for this study. The methanol extract of Blumea lanceolaria exhibited significant DPPH radical scavenging property with an IC 50 of 45.79µg/ml whereas the IC 50 value of the standard
more » ... of the standard drug L-ascorbic acid was found to be 13.76µg/ml. The methanol extract also exhibited significant antilipid peroxidative effect in Fe 2+-ascorbate system. The IC 50 value of the extract was found to be 62.48µg/ml, where as the standard drug exhibited 32.64µg/ml. The extract significantly scavenged the hydroxyl radicals by Fe 3+-ascorbate system generated through the Fenton's reaction and it has also significant reducing ability at IC 50 of 31.78µg/ml. The extract also exhibited significant nitric oxide radical scavenging property at IC 50 of 80.85µg/ml. In the estimation of total phenolics was found that one milligram of the extract contains 59.03µg equivalent of pyrocatechol. Thus from the present investigation it can be concluded that the defatted methanol extract of Blumea lanceolaria exhibited significant antioxidant property in various invitro system.
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