Resistance of oxidative stress in biofilm and planktonic cells

Witold Jakubowski, Bogdan Walkowiak
2015 Brazilian Archives of Biology and Technology  
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H 2 O 2 , tBOOH, menadione, SIN-1 or peroxynitrite) in a wide
more » ... rangeof finalconcentrations, from0.5 to 10mM.Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in logarithmic phase of growth treated with H 2 O 2 , both in planktonic and biofilm forms, whereas for the cells in stationary phase, the highest resistance was observed for menadione. These results showed higher efficiency of agents based on superoxide anion donors in combating bacteria colonizing abiotic surfaces stainless steel (AISI 316L).
doi:10.1590/s1516-8913201500356 fatcat:snllk7qq7rghzg2y7w4bkx2qmq