ENCAPSULATION AND RELEASE PROFILE OF PROTEIN CAGE FROM A POLYMERIC MATRIX

YAN LI, RIKE OKTAVIANTI TOYIP, TAO PENG, SIERIN LIM
2012 Nano LIFE  
Protein cages have been widely investigated as molecular drug carrier. E2 protein from Bacillus stearothermophillus forms a dodecahedral cage structure of approximately 24 nm in diameter. To formulate a sustainable release pro¯le, E2 protein was further encapsulated into poly(lactide-coglycolide) (PLGA) microparticles to form a composite structure using water-in-oil-in-water (W/O/W) double emulsion method. The in°uence of fabrication parameters on microparticle morphology and E2 protein release
more » ... E2 protein release pro¯le were investigated. The microparticle size increased when the stirring speed of the second emulsi¯cation decreased. Decrease in the volume of external aqueous phase led to the reduction of microparticle size without a®ecting its porosity. The higher ionic concentration of external aqueous phase in the presence of surfactant resulted in microparticles with closed pores on surface. Increase in polymer concentration also led to the formation of less porous microparticles. The E2 protein was not dissociated upon encapsulation into PLGA microparticles based on the unchanged particle size of E2 protein. E2 protein release was studied in phosphate-bu®ered saline solution at 37 C. The initial burst and release rate were lowered as the surfactant concentration in external water phase during the fabrication process was increased from 0.1% to 1% (w/v). After 14-day incubation, no observable polymer degradation was found while the surface of microparticles appeared to be smoother than before incubation.
doi:10.1142/s1793984411000347 fatcat:uytls467jbgmlljjsl7jhqdvom