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Production of Phenylpyruvic Acid by Engineered L-Amino Acid Deaminase from Proteus Mirabilis
[post]
2021
unpublished
Objectives This study aimed to develop an efficient enzymatic strategy for the industrial production of phenylpyruvate (PPA) from L-phenylpyruvic acid (L-Phe). Results L-amino acid deaminase from Proteus mirabilis (L-pmAAD) was expressed in E. coli BL21(DE3) and modified to release product inhibition by employing conformational dynamics engineering. Based on structural analysis, two residues (E145A/L341A) were identified for reducing interactions between the product and enzyme and increasing
doi:10.21203/rs.3.rs-1045341/v1
fatcat:tnsmnpiatfgwvnrroc55y4hxom