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Comet assay is a widely used method, especially in the field of genotoxicity, to quantify and measure DNA damage visually at the level of individual cells with high sensitivity and efficiency. Generally, computer programs are used to analyze comet assay output images following two main steps. First, each comet region must be located and segmented, and next, it is scored using common metrics (e.g., tail length and tail moment). Currently, most studies on comet assay image analysis have adopteddoi:10.1038/s41598-020-75592-7 pmid:33144610 pmcid:PMC7609680 fatcat:pp3fvg22infclkspsruhg5iiei