Optimization of total phenolic extraction of Chromolaena odorata leaf for antifungal activity against plant pathogens

Biet V. Huynh
2019 ˜The œJournal of Agriculture and Development  
Phenolic compounds are secondary metabolites mainly responsible for different pharmacological activities of the plant extracts. In this study, microwave-assisted extraction (MAE) variables were optimized for the extraction of the phenolic compounds from Chromolaena odorata (C. odorata) by using a central composite design (CCD) of response surface methodology (RSM). The optimized conditions were at 62oC for 3 min with solvent to feed ratio of 14:1 (mL/g) and ethanol concentration of 50% (v/v).
more » ... e highest yield of total phenolic compounds was 75.76 mgGAE/g. Ethanol extract clearly showed antifungal activity through antifungal index. The antifungal effect Phytophthora capsici, Fusarium oxysporum and Colletotrichum siamese were 79.07%, 78.66% and 78.42%, in 0.5% of ethanol extract, respectively
doi:10.52997/jad.6.06.2019 fatcat:hsbjyzcr4bcxropqq5dgjvhfzq