Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and Se-Click reaction

Ming-Jie Han, Qing-Tao He, Mengyi Yang, Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kongkai Zhu, Chang-Xiu Qu, Fan Yang, Cheng Hu (+5 others)
2021 Chemical Science  
Single-molecule Förster resonance energy transfer (smFRET) is a powerful tool for investigating the dynamic properties of biomacromolecules. However, the success of protein smFRET relies on the precise and efficient labeling...
doi:10.1039/d1sc02653d pmid:34276941 pmcid:PMC8261736 fatcat:s5tje4uj2ve7nlqxvwy5mtscpq