Skeletal myoblast implants induce minor propagation delays, but do not promote arrhythmias in the normal swine heart

Javier Moreno, Jorge G. Quintanilla, Antonio López-Farré, Tamara Archondo, Raquel Cervigón, Paloma Aragoncillo, Elena Usandizaga, Jacobo Silva, Cruz Rodríguez-Bobada, José Luis Rojo, Nicasio Pérez-Castellano, Sergey Mironov (+4 others)
2010 Europace  
Aims Whether skeletal myoblast (SM) implants are proarrhythmic is still controversial due to conflicting pre-clinical and clinical data. We hypothesized that if SM implants are arrhythmogenic, they will facilitate the induction of ventricular tachyarrhythmias by promoting heterogeneous propagation of activation wavefronts. Methods Skeletal myoblast cells were harvested from 10 pigs. A month later, 125 + 37 × 10 6 cells were subepicardially injected in an area of 2 cm 2 at the anterolateral
more » ... t of the left ventricle. Four weeks later, a ventricular stimulation protocol was conducted. Once explanted, epicardial wavefronts over SM and adjacent control areas were optically mapped. Eight saline-injected animals were used as controls. To compare with clear arrhythmogenic substrates, propagation patterns were also evaluated in infarcted hearts and on a SM-implanted heart following amiodarone infusion. Results In SM hearts, fibrosis and differentiated SM cells were consistently found and no tachyarrhythmias were induced. Wavefronts propagated homogeneously over SM and adjacent areas, with no late activation zones, as opposed to the infarcted hearts. The time required for the wavefronts to depolarize both areas were similar, becoming only slightly longer at SM areas after an extra-stimulus (P ¼ 0.025). Conduction velocities and APD 90 were also similar. Saline hearts showed similar results. The extent of the conduction delay was not related to the number of injected SM cells. Conclusion In normal swine hearts, myoblast implants promote localized fibrosis and slightly retard epicardial wavefront propagation only after extra-stimuli. However, SM implants are not associated with local re-entry and do not facilitate ventricular tachyarrhythmias in the whole normal heart. ---
doi:10.1093/europace/euq278 pmid:20675673 fatcat:c467h77wkjfjfll5qvbykxrls4