Characterizing mechanics of membrane tether from relaxation curve obtained by optical tweezers [article]

Xuanling Li, Xiaoyu Song, Yinmei Li, Ming Li, Haowei Wang
2020 bioRxiv   pre-print
Optical tweezers is a powerful tool in the study of membrane tension. Comparing to pulling out an entire membrane tether at one time, the step-like method is more efficient because multiple relaxation curves can be obtained from one membrane tether. However, there is few proper models that describe relaxation curves to characterize mechanical properties of cell membrane. Here we established a model to describe the relaxation curve of HeLa cells based on the relationship between membrane tether
more » ... iameter and tensions. We obtained effective viscosities and static tensions by fitting relaxation curves to our model. We noticed the delicate structure of relaxation curves contains information of cell skeleton changes and protein diffusion. Our study paved a novel pathway to characterize the dynamics and mechanics of cell membrane.
doi:10.1101/2020.09.06.285353 fatcat:qpvp7fljkvf7rpnauu6my6ki4e