Deletion of Irf3 and Irf7 Genes in Mice Results in Altered Interferon Pathway Activation and Granulocyte-Dominated Inflammatory Responses to Influenza A Infection

Bastian Hatesuer, Hang Thi Thu Hoang, Peggy Riese, Stephanie Trittel, Ingo Gerhauser, Husni Elbahesh, Robert Geffers, Esther Wilk, Klaus Schughart
2016 Journal of Innate Immunity  
strong increase of mortality associated with a massive influx of granulocytes in the lung and reduced activation of the adaptive immune response. Fig. 6. Quantification of IFN type I and type III protein levels in KO and WT mice infected with PR8M IAV. After intranasal infection of female mice at the age of 8-11 weeks (n = 5-8) with PBS or 2 × 10 5 FFU PR8M, BAL was collected at the indicated days and used for IFNA ( a ), IFNB1 ( b ) or IFNL2/3 (IL-28A/B; c ) detection by ELISA. Each sample was
more » ... measured in technical duplicates. Values from individual mice, the mean and SEM are presented. The blue line indicates the detection limit of each specific assay. Protein measurements that were below the detection limit were set to the detection limit. The significance of differences in protein concentrations between mutant mice and WT controls were calculated at each time point by the two-tailed
doi:10.1159/000450705 pmid:27811478 fatcat:ephibb7k4revlbicc7f6qpjgbm