3.2.2 Toward functional engineered tissues as biosensors using hydrogels and dielectrophoretic technique

Javier Ramón-Azcón, Samad Ahadian, Serge Ostrovidov, Vahid Hosseini, Ali Khademhosseini, Tomokazu Matsue, Raquel Obregón, Kosuke Ino, Hitoshi Shiku, Gulden Camci-Unal, AMA Association For Sensor Technology E.V., Sophie-Charlotten-Str. 15, 14059 Berlin, Germany
Microscale technologies have been emerged as powerful tools for tissue engineering. Such technologies render precise positioning for the cells in order to define the cell-cell and cell-extracellular matrix (ECM) interactions mimicking the structure of native tissue constructs. Dielectrophoresis (DEP) method is a suitable microscale technology to do that having notable characteristics in cell manipulation such as being high accurate, rapid, scalable, and capable of handling both adherent and
more » ... th adherent and non-adherent cells. DEP could be used in combination with new biomaterials to embed the cells within a given pattern, allowing precise positioning of cells in order to define interactions between neighboring cells. In this investigation, we propose the gelatin methacrylate (GelMA) as a promising hydrogel for the cell dielectropatterning due to low viscosity and ionic concentration. Combined application of the GelMA hydrogel and DEP technique could be useful for precisely creating complex and cellresponsive microtissues in a rapid, accurate, and scalable manner. In this study, we proposed also the interdigitated array of electrodes as a novel platform to electrically stimulate the 3D engineered muscle tissue. The attained muscle myofibers were analyzed and quantified in terms of myotube characteristics and gene expression. These engineered tissues have the potential to serve as biosensors to examine biologically active reagents and could be applied to pharmacological screening and environmental monitoring.
doi:10.5162/imcs2012/3.2.2 fatcat:ffownx5shve2nn7juf2zr55amq