Effect of probe pressure on cervical fluorescence spectroscopy measurements

Audrey Nath, Kelley Rivoire, Sung Chang, Dennis Cox, E. Neely Atkinson, Michele Follen, Rebecca Richards-Kortum
2004 Journal of Biomedical Optics  
Fluorescence spectroscopy is a promising technology for detection of epithelial precancers and cancers. While age and menopausal status influence measurements in the cervix, other variables do not significantly affect the diagnosis. In this study we examine probe pressure as a variable. A fiber optic probe to measure fluorescence spectra at different calibrated levels of pressure was designed and tested. A pilot study was conducted measuring fluorescence excitation emission matrices in 20
more » ... ts at light, medium, and firm pressure. Spectroscopic data were pre-processed and analyzed to compare mean peak intensities as a function of pressure. Further statistical analyses tested for differences in intensities at each excitation/ emission wavelength pair. Four providers made measurements from 41 sites; 33 yielded good quality spectroscopic data (22 squamous normal, 7 squamous abnormal, 3 columnar normal, 1 transformation zone) from 17 of 20 patients. At all pressure levels, abnormal tissue showed less fluorescence intensity than normal tissue, and postmenopausal patients showed higher fluorescence intensity than premenopausal patients, consistent with previous analyses. A permutation analysis suggests that pressure does not significantly affect fluorescence intensity or lineshape. While other studies are needed to confirm these findings, this study suggests that fluorescence spectroscopy is a robust technology likely not influenced by fiber optic probe pressure. Downloaded From: http://biomedicaloptics.spiedigitallibrary.org/ on 04/03/2014 Terms of Use: http://spiedl.org/terms Fig. 5 Mean peak intensity at all three pressure (light, medium, and firm) levels from 17 squamous sites in pre-menopausal women and 12 squamous sites in post-menopausal women at (a) 320-nm excitation, (b) 380-nm excitation, and (c) 470-nm excitation. Effect of probe pressure .
doi:10.1117/1.1695562 pmid:15189090 fatcat:4azqghbbwjhtjc7jlzmgt3oetq