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We analysed the responses of Anabaena doliolum to elevated levels of copper and zinc in batch and semi-continuous cultures. Approximately 10, 4 and 8 and 5-times greater inhibition in final yield of A. doliolum occurred at 1, 2 mM Cu and 2.5 and 5 mM of Zn, respectively, in semi-continuous culture in comparison to batch culture. Protein, chlorophyl a and carotenoid contents of A. doliolum showed significantly (P < 0.05) higher inhibition by test metals in semi-continuous culture than in batchdoi:10.1051/limn/2009010 fatcat:pojzqnvwgjbmxnw5dygbj4ekve