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In a previous article, it was reported that, under stress conditions caused by Ca 2q ions, isolated protoplasts of Betula platyphylla leaves release a bundle of hollow fibrils as a stress-induced response. In the present article, details of this phenomenon have been investigated. As a special method, light microscopy combined with a 'through focus variation' technique was applied. In a regular culture medium, protoplasts could regenerate the cell walls, whereas protoplasts submitted to Ca 2qdoi:10.1515/hf.2011.158 fatcat:23fekboo3rdr7ihvqpmidnwzdq