Morphological responses of Betula protoplasts in fiber spinning

Tomoko Seyama, Tetsuo Kondo
2012 Holzforschung  
In a previous article, it was reported that, under stress conditions caused by Ca 2q ions, isolated protoplasts of Betula platyphylla leaves release a bundle of hollow fibrils as a stress-induced response. In the present article, details of this phenomenon have been investigated. As a special method, light microscopy combined with a 'through focus variation' technique was applied. In a regular culture medium, protoplasts could regenerate the cell walls, whereas protoplasts submitted to Ca 2q
more » ... bmitted to Ca 2q stress could not. Under stress conditions, the protoplasts inflated and formed a huge vacuole and, after 28 days, secreted the peculiar gigantic fibril with a diameter of 150 mm. The giant fibril is composed of (1™3)-b-glucan chains, i.e., the material of callose tissue.
doi:10.1515/hf.2011.158 fatcat:23fekboo3rdr7ihvqpmidnwzdq