1P110 Effect of partial fluorination on bacteriorhodopsin reconstituted in dimerized Di-o-tetradecylphosphatidylcholine vesicle(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Naoyuki Tsuchida, Toshiyuki Takagi, Takashi Kikukawa, Hiroshi Takahashi, Toshiyuki Kanamori, Masashi Sonoyama
2014 Seibutsu Butsuri  
Optogenetics has revolutionized neurosciences, where ion-transporting microbial rhodopsins are utilized as tools for light-induced neuronal excitation and suppression. Channelrhodopsin (ChR), a light-gated cation channel, is used to excite neurons by light. Recently, several chimeric proteins have been designed for better optogenetics application. Here we applied low-temperature spectroscopy to the chimeric ChR, ReaChR (Red-activatable ChR). We found accumulation of photointermediates at low
more » ... peratures by UV-vis spectroscopy. Lightinduced difference FTIR spectra were measured between dark state and photointermediates in the presence of various cations. Structural changes of ReaChR and its cation effects will be discussed. 1P110 Effect of partial fluorination on bacteriorhodopsin reconstituted in dimerized Di-o-tetradecylphosphatidylcholine vesicle
doi:10.2142/biophys.54.s159_2 fatcat:echzmqtff5b6dmdhava37b34ii