Re-sequencing and transcriptomic analysis reveals rich DNA sequence variation and differential gene expression between varieties of Ziziphus jujuba [post]

Na Li, Yuqin Song, Jie Li, Ruijie Hao, Xinxin Feng, Liulin Li
2021 unpublished
Background: Jujube is one of the characteristic fruit tree species in China. 'Linhuang No. 1', a cracking-resistant cultivar, and 'Muzao', a cracking-susceptible cultivar, were selected as materials by previous study. Whole-genome re-sequencing and transcriptome of 'Linhuang No. 1' and 'Muzao' allow the screening out of differently expressed genes with different gene structures between them. It could be helpful in explaining divergence/similarity of cracking resistance between the two
more » ... Results: There are 664,129 mutation sites between 'Linhuang No. 1' and 'Muzao' by re-sequencing. To determine the genetic relationship of 'Linhuang 1', 'Muzao' and reference genome 'Dongzao', the characteristic mutation sites were analyzed by principal component analysis. The genetic relationship between 'Linhuang No. 1' and 'Muzao' was closer than that with 'Dongzao'. A total of 431 differentially expressed genes was screened by transcriptomics, and 19 differentially expressed genes were screened by combining the transcriptomics with re-sequencing analysis. LOC107427052 (encoding nitrite reductase) was determined by KEGG enrichment analysis for further study. Conclusions: The large base insertion was not in the domain region of the LOC107427052 gene CDS region. As verified by the finding that the base insertion did not affect protein translation. Our study has laid a foundation for the analysis of genetic information and the comparative nitrite metabolism of 'Linhuang No. 1' and 'Muzao'.
doi:10.21203/rs.3.rs-253917/v1 fatcat:s3t5ccfuafbdjnjr3gke44wviq