Low-volume multiplexed proteolytic activity assay and inhibitor analysis through a pico-injector array

Ee Xien Ng, Miles A. Miller, Tengyang Jing, Doug A. Lauffenburger, Chia-Hung Chen
2015 Lab on a Chip  
In this study we developed a pico-injector array to generate 9 × 2 × 2 × 2 = 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple protease reactions and measurement of inhibitor effects by using small sample volumes.
doi:10.1039/c4lc01162g pmid:25553996 fatcat:wmkng45hpbhsjpxi7cnff2rqp4