In this project, the prevalence of mastitis cases, isolation, identification and antimicrobial susceptibilities of S. aureus and E. coli causing mastitis were aimed in the center and districts of the province of Kutahya. Different races of 350 cows were milked at different lactation periods and 111 milk samples (31.71%) inspected by California Mastitis Test (CMT) were recorded as positive. S. aureus were isolated from 60 milk samples whereas E. coli were isolated from 51 samples. Identification

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... was carried out by traditional biochemical tests. Antimicrobial susceptibility tests showed that 45 out of 60 isolates (75%) in S. aureus and 51 out of 43 (84.31) isolates in E.coli were resistant to penicillin. Erythromycin, amoxicillin-clavulanic acid, gentamycin resistance were also determined for S. aureus and E. coli isolates. The study provides valuable information about the measures to be taken and treatment methods that can be applied against mastitis-borne infections contributing to take precautions against the economic losses caused by mastitis in Kutahya.