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Central nervous system infection with free-living amoebae is rare. We present a fatal case of Acanthamoeba encephalitis in a 63-yearold female from India where acanthamoebae were demonstrated and cultured from CSF. In spite of treatment with amphotericin B, ß uconazole and rifampicin the patient did not survive. Amoebic infection should be suspected in a patient of encephalitis of unexplained aetiology as timely diagnosis can lead to a favourable outcome.doi:10.4103/0255-0857.40539 pmid:18445961 fatcat:le7dnkgzujfb3bqmuilj4uhmly