Maternal high fat diet induces impaired polyunsaturated fatty acid synthesis in adult female offspring in rats
Proceedings of the Nutrition Society
The long chain PUFA arachidonic acid (20: 4n -6) and dosocahexanoic acid (22: 6n -3) can be synthesised from their respective essential fatty acid precursors linoleic acid (18 :2n -6) and a-linoleic acid (18: 3n -3) by a series of desaturation and chain elongation reactions (1) . Dietary fat alters capacity for 20 :4n -6 and 22 :6n -3 synthesis in adults (1) . The purpose of this study was to determine whether the type and amount of fat consumed by rats before and during pregnancy, and during
... nancy, and during lactation alters liver PUFA status and mRNA expression of the genes encoding D6 (FADS2) and D5 (FADS1) desaturases in the adult offspring. The study was carried out in accordance with the Home Office Animals (Scientific Procedures) Act (1986). Female Wistar rats (n 5 per group) were fed diets containing either 7% (w/w) or 21 % (w/w) safflower oil (SAO) or fish oil (FO) from 14 d before conception until the offspring were weaned on postnatal day 28. Offspring (n 5 females per maternal diet) were weaned onto AIN93M containing 4 % (w/w) soybean oil (without 20 :4n -6 and 22 : 6n -3) and killed on postnatal day 77 following a 12 h fast. Livers were collected into liquid nitrogen and stored at -80 C. Liver phosphatidylchoine (PC) fatty acid compositions were measured by gas chromatography (2) . FADS 1 and 2 mRNA expression were measured by real-time RTPCR (3) .