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A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3
[article]
2020
bioRxiv
pre-print
A ratiometric genetically encoded voltage indicator (GEVI) would be desirable for tracking transmembrane voltage changes in cells that are undergoing motion. To create a high-performance ratiometric GEVI, we explored the possibility of adding a voltage-independent red fluorophore to ASAP3, a high-gain green fluorescent GEVI. We performed combinatorial multi-site mutagenesis on the cyan-excitable red fluorescent protein mCyRFP1 to enhance brightness and monomericity, creating mCyRFP2. Among red
doi:10.1101/2020.10.09.328922
fatcat:56mqrkyzbrhvxihauuuysogdfa