Comparative larvicidal efficacies of Nymphae lotus fractions against Anopheles mosquito larvae

A.A. Yakubu, A.A. Salisu, D.B. Hassan, T.S. Imam
2018 Bayero Journal of Pure and Applied Sciences  
This study aimed at comparing the larvicidal efficacy of five Nymphaea lotus against Anopheles (Culicidae). Ethanolic extract of the dried leaves of Nymphaea lotus was partitioned with chloroform, methanol, n fractions E001, E002, E003, E004 and E005 respectively. Th the five fractions were tested in triplicates by exposing the larvae to 1000, 500, 250, 125, 62.5 and 31.25µg/ml respectively, the result obtained was subjected to One Way Analysis of Variance and Probit analysis using SP reveals
more » ... using SP reveals the presence of alkaloids, steroid, flavonoids, terpenoids, tannins and saponins. GCMS analysis of the five fractions shows the presence of over forty five (45) pre of interest. The result obtained shows that the n lethal effect against both the Anopheles culicidae mosquito larvae and Brine Shrimp with LC of 81.30 and159µg/ml, respectively.In light of the above findings, the n Nymphaea lotus could be of immense potentiality in the search for a bio the fight against malaria as it has been found to be harmless to other aquatic organisms at lower concentration. This study aimed at comparing the larvicidal efficacy of five extract of the leaves of Nymphaea lotus against Anopheles (Culicidae). Ethanolic extract of the dried leaves of Nymphaea lotus was partitioned with chloroform, methanol, n-hexane and water to yield fractions E001, E002, E003, E004 and E005 respectively. The larvicidal efficacies of each of the five fractions were tested in triplicates by exposing the larvae to 1000, 500, 250, 125, 62.5 and 31.25µg/ml respectively, the result obtained was subjected to One Way Analysis of Variance and Probit analysis using SPSS 16.0. Phytochemical screening of the five fractions reveals the presence of alkaloids, steroid, flavonoids, terpenoids, tannins and saponins. GCMS analysis of the five fractions shows the presence of over forty five (45) pre-cursor compounds . The result obtained shows that the n-hexane fraction (E004) has the highest lethal effect against both the Anopheles culicidae mosquito larvae and Brine Shrimp with LC of 81.30 and159µg/ml, respectively.In light of the above findings, the n-hexane frac Nymphaea lotus could be of immense potentiality in the search for a bio-friendly larvicide for the fight against malaria as it has been found to be harmless to other aquatic organisms at
doi:10.4314/bajopas.v10i1.91s fatcat:fpdp43jffbgiraj5tt5mdtgaye