Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria

Natsumi FUJIWARA, Keiji MURAKAMI, Michiyasu NAKAO, Munehisa TOGUCHI, Hiromichi YUMOTO, Katsuhiko HIROTA, Takashi MATSUO, Shigeki SANO, Kazumi OZAKI, Yoichiro MIYAKE
2017 Journal of oral health and biosciences  
キーワード:Lactobacillus reuteri, reuterin, Fusobacterium nucleatum, biofilm Abstract:OBJECTIVE: Lactobacillus reuteri is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by L. reuteri and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related
more » ... reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria. MATERIALS AND METHODS: The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by Fusobacterium nucleatum were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes. RESULTS: Among synthesized RRCs, RRC-, -, -, -, -, -, and -, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-, -, -, and -, significantly suppressed biofilm formation to -% of the control. Most RRCs, except for RRCand -, were not cytotoxic to human oral keratinocytes. CONCLUSIONS: The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.
doi:10.20738/johb.30.1_8 fatcat:zosnvsjtobajlhgjwsr6v6dnta