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In order to evaluate rumen microbial activity, the effects of two nitrogen sources (organic and inorganic) were analyzed in vitro by inoculating dairy cow rumen fluid and using citrus byproduct (CBP) and milk thistle (Silybum marianum, MT) as energy source substrates. The experimental design was 2 × 3 where we used three different nitrogen sources (N1: no nitrogen added, N2: ammonium bicarbonate, N3: sodium glutamate) with a basic concentration of nitrogen (100 mg/1 L). Culture pH, dry matterdoi:10.3906/vet-1710-32 fatcat:atyosgoh7vecxoq2ug46esqsqm