Comparative study between standard pyocyanin and extracted pyocyanin and synergistic with antibiotics against multidrug bacteria isolated from different clinical samples

Sabreen H. Dhairh, Ibtisam H. Al-Azawi
2022 International Journal of Health Sciences  
Multidrug Resistance forms in Gram positive and Gram negative bacteria are difficult to treat, and medications to be taken with caution may be unsuccessful. The emergence of treat MDR Gram-negative and Gram-positive Infections bacterial is still a major issue. Aims: The goal of this study is reliable develop new agents or new treatment methods that are capable of overcoming drug resistance in these organisms. Methods: 536(85.3%) samples from out and inpatients who admitted to Al Diwaniyah
more » ... ng Hospital in Diwaniyah city were collected from various site of infection , 92 (46%) Pseudomonas aeruginosa were investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Nutrient agar) to detect , Pseudomonas aeruginosa , the samples were identified using the Vitec 2 compact system, Pyocyanin was extracted by standard methods from Pseudomonas aeruginosa Principal antimicrobial compounds present in pyocyanin were identified by HPLC . Other pathogenic bacteria obtained for exposure to Pyocyanin like Escherichia coli , K. pneumoniae , S. aureus from laboratories These diagnosis was confirmed by Vitek 2 system . Serratia marcescens and Proteus mirabilis obtained from sputum patients with co- infection Covid 19.
doi:10.53730/ijhs.v6ns6.10274 fatcat:4akl6432lbfjzgd36v3cavaece