Multidrug Resistance forms in Gram positive and Gram negative bacteria are difficult to treat, and medications to be taken with caution may be unsuccessful. The emergence of treat MDR Gram-negative and Gram-positive Infections bacterial is still a major issue. Aims: The goal of this study is reliable develop new agents or new treatment methods that are capable of overcoming drug resistance in these organisms. Methods: 536(85.3%) samples from out and inpatients who admitted to Al Diwaniyah

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... ng Hospital in Diwaniyah city were collected from various site of infection , 92 (46%) Pseudomonas aeruginosa were investigated by a qualified consulting doctor. After cultivation in special media (MacConkey agar and Nutrient agar) to detect , Pseudomonas aeruginosa , the samples were identified using the Vitec 2 compact system, Pyocyanin was extracted by standard methods from Pseudomonas aeruginosa Principal antimicrobial compounds present in pyocyanin were identified by HPLC . Other pathogenic bacteria obtained for exposure to Pyocyanin like Escherichia coli , K. pneumoniae , S. aureus from laboratories These diagnosis was confirmed by Vitek 2 system . Serratia marcescens and Proteus mirabilis obtained from sputum patients with co- infection Covid 19.