Article Highlights • Protein profile of domestic Balkan donkey milk during lactation period to the 280 th day were determined • Donkey's milk protein profiles were determined by applying lab-on-a-chip electrophoresis • Domestic Balkan donkey milk was found to be low in casein content • Lysozyme, lactoferrin and immunoglobulins were identified • Balkan donkey milk represents a rich source of high nutritive components Abstract Domestic Balkan donkey (Equus asinus asinus) is a native donkey breed,

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... primarily found in the northern and eastern regions of Serbia. The objective of the study was to analyze proteins of Domestic Balkan donkey milk during the lactation period (from the 45 th to the 280 th day) by applying lab-on-a-chip electrophoresis. The chip-based separations were performed on the Agilent 2100 Bioanalyzer in combination with the Protein 80 Plus lab chip kit. The protein content of domestic Balkan donkey milk during the lactation period of 280 days ranged from 1.40 to 1.92% and the content of αs1-casein, αs2-casein, β-casein, α-, β-lactoglobulin, lysozyme, lactoferrin and serum albumin was relatively quantified. Lysozyme (1040-2970 mg/L), α-lactalbumin 12 kDa (1990-2730 mg/L) and α-lactalbumin 17.7 kDa (2240-3090 mg/L) were found to be the proteins with the highest relative concentrations.