Effect of Sesamin on Apoptosis and Cell Cycle Arrest in Human Breast Cancer MCF-7 Cells

An-Ci Siao, Chien-Wei Hou, Yung-Hsi Kao, Kee-Ching Jeng
2015 Asian Pacific Journal of Cancer Prevention  
Dietary prevention has been known to reduce breast cancer risk. Sesamin is one of the major components in sesame seeds and has been widely studied and proven to have anti-proliferation and anti-angiogenic effects on cancer cells. In this study, the influence of sesamin was tested in the human breast cancer MCF-7 cell line for cell viability (MTT assay) and cell cycling (flow cytometry). Results showed that sesamin dose-dependently (1, 10 and 50 μM) reduced the cell viability and increased LDH
more » ... lease and apoptosis (TUNEL assay). In addition, there was a significant increase of sub-G1 phase arrest in the cell cycle after sesamin treatment. Furthermore, sesamin increased the expression of apoptotic markers of Bax, caspase-3, and cell cycle control proteins, p53 and checkpoint kinase 2. Taken together, these results suggested that sesamin might be used as a dietary supplement for prevention of breast cancer by modulating apoptotic signal pathways and inhibiting tumor cell growth. Cell lines and culture Human breast cancer MCF-7 cell line was purchased from ATCC. Cells were cultured in a humidified incubator at 37°C and 5% CO 2 atmosphere in DMEM (Dulbecco's modified Eagle's medium), supplemented with 10% FBS and 1% penicillin-streptomycin. Cell viability assay Cell viability was measured using blue formazan An-Ci Siao et al
doi:10.7314/apjcp.2015.16.9.3779 fatcat:6gdspdifcjgzjhbqtfrcfebpw4