Risk factors and detection of brucella ovis in naturally infected rams and ewes

Letícia Soares de Araújo Teixeira, Flaviane Alves de Pinho, Joilson Ferreira Batista, Misael das Virgens Santana, Francisco Felipe Ferreira Soares, Ana Milena César Lima, Leonardo Lopes Furtado, Francisca Kelly dos Santos Silva, Luma Martins Nunes Santos, Tuanny Creusa Medeiros Damasceno, João Farias de Sousa Junior, Marlene Sipaúba Oliveira (+3 others)
2021 Revista Brasileira de Ciência Veterinária  
The objective of this study was to verify the occurrence of ovine brucellosis using Agar Gel Immunodiffusion (AGID) and Polymerase Chain Reaction (PCR) techniques, as well as to identify the main risk factors associated with infection in sheep flocks belonging to municipalities in the microregion from Teresina, PI, Brazil. A total of 100 urine and blood samples were collected from sheep aged 6 months or older. The urine samples were submitted to conventional PCR and the blood samples were
more » ... ed by the AGID technique. Of the 100 blood samples, 17 (17%) were reactive to the AGID test. In conventional PCR of 100 urine samples, six (6%) were positive. Risk factors associated to infection by B. ovis included the rearing system (OR=0.19), feed management (OR=0.05), presence of dystotic births (OR=4.50), miscarriages (OR=3.75) and source of water offered to the animals (OR=0.19). Thus, it was concluded that it is possible to detect the occurrence of animals with ovine brucellosis since PCR is a reliable method to confirm infection. Furthermore, there are risk factors associated to infection by B. ovis in the municipalities studied.
doi:10.4322/rbcv.2021.009 fatcat:6mffsksqxjcxnazwed5xhn6pru