RAPD ANALYSIS IN ANDROGRAPHIS PANICULATA (BURM. F.) NESS PLANT TYPES

Benoy K Ghosh, Aninda Mandal, Animesh K Datta, Debadrito Das
2014 International Journal of Research in Ayurveda and Pharmacy  
RAPD (random amplification of polymorphic DNA) markers have been used to elucidate genetic distinctiveness between/among 11 (selfed control lines and 10 true breeding M4 macro mutant lines) plant types of Andrographis paniculata (Burm. F.) Ness (Family: Acanthaceae; medicinal plant species with immense therapeutic uses). Out of the 30 RAPD primers, 25 reproduced a total of 240 bands of which 170 (70.83 %) were polymorphic. The average number of fragments amplified by RAPD markers across the
more » ... kers across the plant types has been 9.6 with a range of 5 to 19 (size 190 bp to 1065 bp). RAPD primers studied across the plant types varied in relation to scorable fragments, polymorphism percentage (28.57 % to 90.91 %), polymorphism information content -PIC (0.07513 to 0.47230) and marker index -MI (3.41 to 36.34). Results obtained for Shannon diversity index -I (2.3514 to 2.3985), genetic diversity/locus -Hep (0.904 to 0.911) and effective allele/locus -Aep (10.41 to 11.23) suggested narrow genetic base of the studied plant types. Band spectra analyzed by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed 6 major clusters and a close relatedness between/among the plant types, which may be significant for designing efficient breeding programme towards crop improvement. Upon considering different genetic parameters, taken together, it seems that OPA 06, OPA 08-09, OPB 05, OPC 01-04, OPC 06 and OPC 09 are efficient and effective RAPD primers for molecular screening of A. paniculata germplasms.
doi:10.7897/2277-4343.05117 fatcat:yoxovrx42rhxbi5msil634zvjy