RAPD ANALYSIS IN ANDROGRAPHIS PANICULATA (BURM. F.) NESS PLANT TYPES
International Journal of Research in Ayurveda and Pharmacy
RAPD (random amplification of polymorphic DNA) markers have been used to elucidate genetic distinctiveness between/among 11 (selfed control lines and 10 true breeding M4 macro mutant lines) plant types of Andrographis paniculata (Burm. F.) Ness (Family: Acanthaceae; medicinal plant species with immense therapeutic uses). Out of the 30 RAPD primers, 25 reproduced a total of 240 bands of which 170 (70.83 %) were polymorphic. The average number of fragments amplified by RAPD markers across the
... kers across the plant types has been 9.6 with a range of 5 to 19 (size 190 bp to 1065 bp). RAPD primers studied across the plant types varied in relation to scorable fragments, polymorphism percentage (28.57 % to 90.91 %), polymorphism information content -PIC (0.07513 to 0.47230) and marker index -MI (3.41 to 36.34). Results obtained for Shannon diversity index -I (2.3514 to 2.3985), genetic diversity/locus -Hep (0.904 to 0.911) and effective allele/locus -Aep (10.41 to 11.23) suggested narrow genetic base of the studied plant types. Band spectra analyzed by UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed 6 major clusters and a close relatedness between/among the plant types, which may be significant for designing efficient breeding programme towards crop improvement. Upon considering different genetic parameters, taken together, it seems that OPA 06, OPA 08-09, OPB 05, OPC 01-04, OPC 06 and OPC 09 are efficient and effective RAPD primers for molecular screening of A. paniculata germplasms.