Validation Study on a Method for Multiresidue Analysis of Pesticides in Cereals and Pulses with Supercritical Fluid Extraction
超臨界流体抽出(SFE)による穀類・豆類中の残留農薬の一斉分析法の妥当性評価

Katsushige URANISHI, Hirokazu YAMASHITA, Akiko OKAYAMA, Keigo YAMAMOTO
2012 Shokuhin Eiseigaku Zasshi  
Supercritical fluid extraction SFE was applied to extraction of pesticides from cereals and pulses. Residues were extracted from homogenized samples mixed with water-absorbent polymer and supercritical carbon dioxide in a stainless steel tube, followed by elution with acetonitrile. Coextractives were removed by means of mini-column clean-up. Measurement was performed by GC-MS/MS. Calibration was achieved by preparing matrix-matched calibration standards to counteract matrix effects. With the
more » ... ffects. With the Japanese method validation guideline for pesticide residues as a reference, the method was assessed in 5 agricultural products spiked with 334 pesticides at 0.01 and 0.1 μg/g. Compounds at each level were extracted from 2 samples on 5 separate days. The trueness of the method for 137 pesticides in all samples was 70-120 , and the repeatability and within-run reproducibility were also consistent with the guideline. The trueness of the method for the other 101 pesticides was in the range of 50-70 , though the repeatability and within-run reproducibility were satisfactory. This method is available as a multiresidue analysis method for cereals and pulses.
doi:10.3358/shokueishi.53.278 pmid:23470855 fatcat:pwgnpxaslvegtg7eic2xudrd4i