Structural DNA Nanotechnology [book]

Nadrian C. Seeman
2015 unpublished
DNA nanotechnology uses reciprocal exchange between DNA double helices or hairpins to produce branched DNA motifs, like Holliday junctions, or related structures, such as double crossover (DX), triple crossover (TX), paranemic crossover (PX) and DNA parallelogram motifs. We combine DNA motifs to produce specific structures by using sticky-ended cohesion (below, left). The strength of sticky-ended cohesion is that it produces predictable adhesion combined with known structure. From simple
more » ... From simple branched junctions, we have constructed DNA stickpolyhedra, such as a cube (below, right) and a truncated octahedron, several deliberately designed knots, and Borromean rings. We have used two DX molecules to construct a DNA nanomechanical device by linking them with a segment that can be switched between left-handed Z-DNA and right-handed B-DNA. PX DNA has been used to produce a robust sequencedependent device that changes states by varied hybridization topology.
doi:10.1017/cbo9781139015516 fatcat:msisbtpzwvh57nywxseslcaoqu