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In this report, we investigate the efficiency and selectivity of a Zn 2+ -dependent peptide nucleic acid-based artificial ribonuclease (PNAzyme) that cleaves RNA target sequences. The target RNAs are varied to form different sizes (3 and 4 nucleotides, nt) and sequences in the bulge formed upon binding to the PNAzyme. PNAzyme-promoted cleavage of the target RNAs was observed and variation of the substrate showed a clear dependence on the sequence and size of the bulge. For targets that formdoi:10.3390/molecules22111856 pmid:29109368 fatcat:l3rjflvupfbf7lepq2mdpartc4