Attenuating Effect of Spirulina Platensis and Alpha-lipoic acid against Aspartame induced Oxidative stress and Brain damagein Rabbits
Benha veterinary medical journal
ARTICLE INFO ABSTRACT Keywords This study was conducted to evaluate the possible protective effect of Spirulina and alphalipoic acid against aspartame induced oxidative stress and brain damage in rabbits. Forty-two white male New-Zealand Rabbits were classified into seven groups. Group I(Control) received no drugs. Group II (ASP) received aspartame (250 mg/kg b. wt./day). Group III (LA)received alpha-lipoic acid (100 mg/kg b. wt./day). Group IV (SPir) received spirulina platensis (1500 mg/kg.
... nsis (1500 mg/kg. b. wt./day). Group V (ASP+ LA) received aspartame (250 mg /kg b. wt.) and alphalipoic acid (100 mg/kg b. wt.). Group VI (ASP+ Spir) received aspartame (250 mg/kg b.wt.) and spirulina platensis (1500 mg/kg b. wt.). Group VII (ASP+ALP+Spir) received aspartame (250 mg/kg b.wt.), alpha-lipoic acid (100 mg/kg b. wt.) and Spirulina platensis (1500 mg/kg b. wt.) for 8 weeks. At the end of experiment, brain tissues were collected and analyzed for Lmalondialdehyde(L-MDA), superoxide dismutase (SOD), reduced glutathione (GSH), gene expression of tumor necrosis factor alpha (TNF-α)and caspase 3, and DNA damage. The results showed a significant up-regulation of TNF-α and caspase 3 gene expression level, marked increase in L-MDA and DNA damage, and a marked decrease in SOD activity and GSH concentration in ASP groupwhen compared with control group. Co-administration of spirulina and alpha-lipoic acid protected aspartame induced brain damage and caused a significant improvement of all previous parameters and attenuated DNA changes. Conclusively, Spirulina platensis and alpha-lipoic acid exerted a protective effect against DNA damage and oxidative stress in the brain of aspartame usage through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidants defense system mechanisms. Alpha-lipoic acid Aspartame DNA damage Oxidative stress Spirulina platensis