Estimation Of Β-Lactamase Activity From Multidrug Resistant Uropathological Bacteria

Archana Moon
2014 Zenodo  
The urinary tract infection (UTI) is a bacterial infection of the urinary system in both community and healthcare settings. β-lactam antibiotics are the typical treatment option for UTI. But the increased rates of antibiotic resistance in bacteria have posed challenges in selecting the antibacterial agent. One of the mechanism bacteria developed to surpass the effect of β – lactam antibiotics is the production and secretion of β-lactamases (EC 3.5.2.6). These antibiotics have a common element
more » ... a common element in their molecular structure, a four-atom ring known as the β-lactam ring. The β-lactamases breaks this ring open, deactivating the molecule's antibacterial property. We have isolated and characterized the bacteria from UTI patients. The isolated bacteria were investigated for the antibiotic susceptibility towards commonly prescribed UTI antibiotics along with β-lactam antibiotics and isolated the multidrug resistant (MDR) clinical isolates. The secretary β-lactamase activities have been checked against eleven β-lactam antibiotics as a substrate by iodometric assay. This method depends upon the reduction of iodine by the hydrolyzed substrate.
doi:10.5281/zenodo.893560 fatcat:qwxezb3egvf7nahymi3tfzipzm