Technical imaging and non-invasive analysis of the areas covered by two paper patches on an Elizabethan map of the coast of North Carolina ('La Virginea Pars': P&D 1906,0509.1.3), drawn by the gentleman artist John White, has revealed a number of changes made to the original design. One patch was used to make minor alterations to the representation of the coastline. The second patch covers a large symbol, which apparently represents a fort or fortification, and bears on its surface a faint version of a similar symbol.