The Effect of Silver Nanoparticles on Biofilm Formation in Environmental Isolates of Pseudomonas Aeruginosa

2020 Indian Journal of Public Health Research & Development  
Alternative therapeutic agents such as silver nanoparticles are considered as a desirable agent to help manage and prevent the biofilm formation of different bacterial species.In the present study, eleven environmental strains of bacteria from water samples were identified as P. aeruginosa using classical method. We synthesized silver nanoparticles (AgNPs) by usingMyrtus communis leaf extract as anti-biofilm formation agent. Using a tube method we have determined the minimum inhibitory
more » ... inhibitory concentrations (MIC) valueof AgNPs against planktonic growth of P. aeruginosa.the AgNPs were exhibited significant inhibition activity against PAE1 (environmental strain) with MICvalue of 150 μg/ml. Moreover, 0.5x MIC of AgNPs were separately tested to examine their anti-biofilm formation activities. We found that 100% (11/11) of environmental isolates were revealed a significant reduction in the rate of the biofilm production in the presence of 0.5x MIC of AgNPs. Thus, these results highlight an inhibitory role for synthesized AgNPs by using Myrtus communis leaf extract on biofilm formation of environmental of P. aeruginosa isolates and suggest that it can be added to the list of a potential anti-biofilm formation targets.
doi:10.37506/ijphrd.v11i4.9130 fatcat:2ytlyljdyfebdfq6qg7snpa42y