ATTENUATION OF GENTAMICIN INDUCED NEPHROTOXICITY IN RATS BY AQUEOUS EXTRACT OF TRIGONELLA FOENUM GRACEUM SEEDS
International Journal of Research in Ayurveda and Pharmacy
The present study was conducted to examine the protective effect of aqueous extract of Trigonella foenum graceum (Fenugreek) on gentamicininduced nephrotoxicity in rats. Aqueous extract of T. foenum graecum was evaluated for its antioxidant property using DPPH and H202 scavenging assay. The nephroprotective effect of plant extract (200, 400 and 800mg/kg p.o.) was tested against gentamicin induced renal injury (100 mg/kg/d i.p.). After 8 days of respective treatment, 24-hour urine was collected
... rine was collected for renal biochemical alterations and blood sample was collected to determine serum creatinine, blood urea nitrogen and serum protein. Kidneys were isolated for antioxidant enzyme estimations and histopathological analysis. Aqueous extract of T. foenum graecum showed antioxidant activity of 77.61 and 71.07% (at 80 µg/ml) in DPPH and H2O2 scavenging assay respectively. Co-administration of plant extract with gentamicin markedly improved all the parameters of nephrotoxicity induced by gentamicin in rats. There was a significant reduction in lipid peroxidation and rise in GSH levels with extract treatment. Histopathological reports showed reduction in the kidney damage in the animals treated with the plant extract. As a conclusion, the aqueous extract of Trigonella foenum graceum seeds may provide a cushion for a prolonged therapeutic option against gentamicin.