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Polymerization of Nonfilamentous Actin into Microfilaments Is an Important Process for Porcine Oocyte Maturation and Early Embryo Development1
Biology of Reproduction
Actin is one of the major proteins in mammalian oocytes. Most developmental events are dependent on the normal distribution of filamentous (F-) actin. Polymerization of nonfilamentous (G-) actin into F-actin is important for both meiosis and mitosis. This study examined G-and F-actin distribution in pig oocytes and embryos by immunocytochemical staining and confocal microscopy. Actin protein was quantified by electrophoresis and immunoblotting. G-Actin was distributed in the whole cytoplasm ofdoi:10.1095/biolreprod62.5.1177 pmid:10775164 fatcat:3hbtgycd2fabvmtb7wrjv7i54y