Investigation of Candidate Genes and Mechanisms Underlying Obesity Associated Type 2 Diabetes Mellitus Using Bioinformatics Analysis and Screening of Small Drug Molecules
BackgroundObesity associated type 2 diabetes mellitus is a metabolic disorder ; however, the etiology of obesity associated type 2 diabetes mellitus remains largely unknown. There is an urgent need to further broaden the understanding of the development mechanism of obesity associated type 2 diabetes mellitus. MethodsTo screen the differentially expressed genes (DEGs) that may play essential roles in obesity associated type 2 diabetes mellitus, the public expression profiling by high throughput
... by high throughput sequencing data (GSE143319) were downloaded and screened for DEGs. Then, Gene Ontology (GO) function analysis and REACTOME pathway analysis were performed. To screen hub and target genes, the protein–protein interaction network, miRNA-target genes regulatory network and TF-target gene regulatory network were constructed. The Receiver operating characteristic (ROC) curve analysis and RT- PCR analysis of hub genes in obesity associated type 2 diabetes mellitus were also analyzed. Final molecular docking studies performed for screening small drug molecules. ResultsThere were 409 up regulated and 411 down regulated genes detected, and the biological processes of the GO analysis were enriched in regulation of ion transmembrane transport, intrinsic component of plasma membrane, transferase activity, transferring phosphorus-containing groups, cell adhesion, integral component of plasma membrane and signaling receptor binding, whereas, the REACTOME pathway analysis was enriched in integration of energy metabolism and extracellular matrix organization. The hub genes CEBPD, TP73, ESR2, TAB1, MAP3K5, FN1, UBD, RUNX1, PIK3R2 and TNF, which might play a essential role in obesity associated type 2 diabetes mellitus was further screened. ConclusionsThe present study could deepen the understanding of the molecular mechanism of obesity associated type 2 diabetes mellitus, which could be useful in developing clinical treatments of obesity associated type 2 diabetes mellitus.