Detection of Tobacco streak virus isolates in North American Cranberry (Vaccinium macrocarpon Ait.)

A. Teifion Jones, Wendy J. McGavin, Alison Dolan
2001 Plant Health Progress  
Tobacco streak virus (TSV) has a wide host range that exceeds 80 species (Fulton, 1948) . Most of the efforts carried out previously comparing TSV isolates was based on immunological relations between them. The isolates of the virus from Fragaria and Rubus have been considered to be very closely related if not identical while they are distinct from isolates of other hosts. While trying to characterize the viruses associated with pallidosis disease, we cloned part of the genome of a virus with
more » ... e of a virus with homology to TSV but distinct enough to be considered a unique virus and not an isolate of TSV. Here we report the complete sequence of RNA 3 of the virus from a strawberry isolate as well as the sequence of the coat protein gene from an additional nine strawberry as well as five Rubus isolates. The data suggest that Fragaria and Rubus are infected with a virus closely related to TSV, designated as Strawberry necrotic shock virus from the name given by Frasier et al. in the first report of the virus infecting strawberry. We were unable to acquire any amplicons utitizing reverse transcription-polymerase chain reaction with primers derived from the published sequence for TSV, an indication that strawberry and Rubus may not be hosts for TSV.
doi:10.1094/php-2001-0717-01-rs fatcat:hxbl3unygralfogxbr5fnqiwky