Aim: To compare the growth factor contents of the various PRP processing methods to get an insight on the difference in growth factor yield from the various processing methods. Experimental: PRP before and after expiry dates was collected from Indonesian Red Cross. PRP was processed by various methods to release the growth factors, and growth factor levels were measured. Growth factor levels between various kinds of processing were compared using ANOVA and those from before and after expiry

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... samples were compared using t test. Results: Comparison of thrombin activation, one, two, and three freeze thaw cycle(s) of all samples revealed no significant difference in PDGF-AB, EGF, and IGF levels (p> 0.05). However, there were significant differences in TGFâ and VEGF levels between thrombin activation and freeze thaw cycles. Comparisons between samples of before and after expiry date revealed no significant differences in all growth factor levels. Conclusion: One, two, and three freeze thaw cycle(s) yielded no significant difference in most growth factor levels.