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Development and Validation of a TLC-Densitometric Method for the Simultaneous Quantitation of Strychnine and Brucine from Strychnos spp. and its Formulations
Journal of Chromatographic Science
A simple, sensitive, and specific thin-layer chromatography densitometric method has been developed for the simultaneous quantitation of strychnine and brucine. These two marker compounds are quantitated in the seeds of Strychnos nux-vomica, Strychnos ignatii, and its formulations. The method involves densitometric evaluation of strychnine and brucine after resolving it by high-performance TLC on silica gel plate with toluene-ethyl acetate-diethyl amine-methanol (7:2:1:0.3 v/v) as the mobiledoi:10.1093/chromsci/45.10.706 pmid:18078581 fatcat:g44ema3kavga7kiihid5cjlm24