Pseudonocardia artemisiae sp. nov., isolated from surface-sterilized Artemisia annua L. A novel actinomycete strain, designated YIM 63587 T , was isolated from surface-sterilized roots of Artemisia annua L. collected from Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 63587 T was affiliated to the genus Pseudonocardia. 16S rRNA gene sequence similarities between strain YIM 63587 T and type strains of species of the genus

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... ardia were 96.6-93.8 %. The diagnostic cell-wall diamino acid in the peptidoglycan layer of strain YIM 63587 T was meso-diaminopimelic acid and the whole-cell sugars were arabinose, galactose, mannose and ribose. The predominant menaquinone was MK-8(H 4 ) (97.7 %). The phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol mannosides, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids (.5 %) were iso-C 16 : 0 (44.7 %), iso-C 14 : 0 (10.3 %), iso-C 16 : 1 H (9.8 %) and iso-C 15 : 0 (7.7 %). The G+C content of the genomic DNA was 68.2 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain YIM 63587 T represents a novel species of the genus Pseudonocardia, for which the name Pseudonocardia artemisiae sp. nov. is proposed. The type strain is YIM 63587 T (5DSM 45313 T 5CCTCC AA 208081 T ).