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The three-dimensional (3D) structure of the wild-type rabbit hemorrhagic disease virus (RHDV) has been determined to a resolution of 3.2 nm by electron cryomicroscopy and computer image reconstruction techniques. The 3D density map exhibits characteristic structural features of a calicivirus: a T=3 icosahedral capsid with 90 archlike capsomeres at the icosahedral and local 2-fold axes and 32 large surface hollows at the icosahedral 5-and 3-fold axes. This result confirms that the RHDV isolateddoi:10.1007/bf03183546 fatcat:kxsmkfwi2bal5b6mwxtdq3uiti