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In Vitro Mutation Induction and Selection of Chrysanthemum (Dendranthema Grandiflora Tzelev) Lines with Improved Resistance to Septoria Obesa Syd
2012
International Journal of Plant Research
This paper describes in vitro mutation selection technique for improved resistance in chrysanthemum (Dendranthema grandiflora Tzelev) cv. Snow Ball against culture filtrate of Septoria obesa, a leaf spot pathogen. The callus was initiated fro m leaf exp lant on MS med iu m supplemented with 10 mg/ l kinetin and 1 mg/l NAA. Opt imal doses of gamma radiation and culture filtrate on the per cent survival of calli were standardized. The optimal dose of gamma radiation was 20 Gy and that of culture
doi:10.5923/j.plant.20120204.01
fatcat:72t7jumyv5cxxcyrekxsntsjnm