Light spectra as triggers for sorting improved strains of Tisochrysis lutea

Fengzheng Gao, Sep Woolschot, Iago Teles Dominguez Cabanelas, René H. Wijffels, Maria J. Barbosa
2020 Bioresource Technology  
It is known that microalgae respond to different light colors, but not at single-cell level. This work aimed to assess if different light colors could be used as triggers to sort over-producing cells. Six light spectra were used: red + green + blue (RGBL), blue (BL), red (RL), green (GL), blue + red (BRL) and blue + green (BGL). Fluorescence-activated cell sorting method was used to analyse single-cell fluorescence and sort cells. BGL and RGBL lead to the highest fucoxanthin production, while
more » ... showed the lowest. Therefore, it was hypothesized that hyper-producing cells can be isolated efficiently under the adverse condition (RL). After exposure to all light colors for 14 days, the top 1% fucoxanthin producing cells were sorted. A sorted strain from RL showed higher (16-19%) growth rate and fucoxanthin productivity. This study showed how light spectra affected single-cell fucoxanthin and lipid contents and productivities. Also, it supplied an approach to sort for high-fucoxanthin or high-lipid cells.
doi:10.1016/j.biortech.2020.124434 pmid:33257166 fatcat:btw74jsjsvddnjyve7mhujt6oe