In VitroAnalysis of the Interaction between the FinO Protein and FinP Antisense RNA of F-like Conjugative Plasmids

Lori J. Jerome, Laura S. Frost
1999 Journal of Biological Chemistry  
EXPERIMENTAL PROCEDURES Generation of DNA Templates for in Vitro Transcription of RNAs-Plasmid pLJ5-13 contains a 105-base pair PCR product with the finP gene fused to the T7 promoter sequence in pUC19 (8). During the construction of pLJ5-13, seven spontaneous mutant clones were obtained with single base mutations in stem II
doi:10.1074/jbc.274.15.10356 pmid:10187824 fatcat:rjizlglsrjdgdksefvlzpw6dom