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In VitroAnalysis of the Interaction between the FinO Protein and FinP Antisense RNA of F-like Conjugative Plasmids
1999
Journal of Biological Chemistry
Preserved Fulltext
EXPERIMENTAL PROCEDURES Generation of DNA Templates for in Vitro Transcription of RNAs-Plasmid pLJ5-13 contains a 105-base pair PCR product with the finP gene fused to the T7 promoter sequence in pUC19 (8). During the construction of pLJ5-13, seven spontaneous mutant clones were obtained with single base mutations in stem II
doi:10.1074/jbc.274.15.10356
pmid:10187824
fatcat:rjizlglsrjdgdksefvlzpw6dom