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Although it is widely appreciated that cells migrate in a variety of diverse environments in vivo, we are only now beginning to use experimental workflows that yield images with sufficient spatiotemporal resolution to study the molecular processes governing cell migration in 3D environments. Since cell migration is a dynamic process, it is usually studied via microscopy, but 3D movies of 3D processes are difficult to interpret by visual inspection. In this review, we discuss the technologiesdoi:10.1016/j.tcb.2015.09.010 pmid:26603943 pmcid:PMC4663125 fatcat:kx5jit4fgraevjhfiskhxtahiy