The experiment was designed to evaluate the effect of sheep manure (SM), its application timing (AT) and N fertilizer (urea) on dry matter partitioning and harvest index. The study was conducted on RCBD split plots arrangement at Agronomic research farm during 2015. Sheep manure (SM1=3 t ha -1 , SM2=4 t ha -1 , SM3=5 t ha -1 ) and application timing (AT1=15 days before sowing, AT2=At sowing time) were allotted to main plots however, fertilizer N (N1=0 kg ha -1 , N2= 90 kg ha -1 , N3=120 kg ha

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... ) were applied to sub-plots. Application of 5 t ha -1 of sheep manure at 15 days before sowing significantly enhanced pre-tasseling (stem and leaves) and physiological maturity (stem, leaves, cobs and grains) dry matter partitioning and harvest index. Pre-tassel and physiological maturity dry matter accumulation were higher with application of 120 kg N ha -1 however, Application of 5 t sheep manure ha -1 at 15 days before sowing and 120 kg N ha -1 was recommended for higher dry matter accumulation of maize crop.