Investigating the role of polymorphisms in miR-146a, -149, and -196a2 in the development of gastric cancer

J.Y. Gu, L. Tu
2016 Genetics and Molecular Research  
Here, we performed a case-control study to investigate the role of miR-146a, miR-149, and miR-196a2 polymorphisms in the development of gastric cancer using a hospital-based case-control design. A total of 186 gastric cancer patients and 186 control subjects were enrolled from Ren Ji Hospital between January 2012 and October 2014. MicroRNAs miR-146a, miR-149, and miR-196a2 were genotyped by polymerase chain reaction coupled with restriction fragment length polymorphism. Univariate logistic
more » ... riate logistic regression analysis revealed that patients with gastric cancer were more likely to be infected with Helicobacter pylori [odds ratio (OR) = 1.68, 95% confidence interval (CI) = 1.07-1.96]. Conditional multiple logistic regression analysis revealed that the TT genotype of miR-196a2 was associated with an increased risk of gastric cancer compared to the CC genotype (OR = 2.40; 95%CI = 1.26-4.61). Moreover, patients carrying both the TC and TT genotypes of miR-196a2 were correlated with an elevated risk of gastric cancer compared to those expressing the CC genotype alone (OR = 1.67, 95%CI = 1.01-2.75; P = 0.03). In conclusion, the results of 2 J.Y. Gu and L. Tu ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (2): gmr.15027839 our study indicated that the miR-196a2 polymorphism was associated with gastric cancer development.
doi:10.4238/gmr.15027839 pmid:27323019 fatcat:alqv7hsqujabtdnxzrnqanjdza